ELOVL6, Stearoyl-CoA desaturase-1 (SCD1), RIP-1, MLKL, and Tumor necrosis factor α (TNF-α) mRNA expression were found to be significantly upregulated in NAFLD patients compared to healthy controls (P < 0.05) (Fig. 6A). This evidence concerns the gene MLKL and metabolic dysfunction-associated steatotic liver disease.