Finally, our data also revealed no change in the expression of CASP1, GSDMD and TGF-β1 proteins, under any of the conditions studied, ruling out a contribution of the inflammasome in this cell death pathway, mediated by the ELOVL6-TNF-α-ROS-RIP-1-MLKL axis, and explaining the absence of inflammation in NAFLD. Here, TGFB1 is linked to metabolic dysfunction-associated steatotic liver disease.