Fibronectin was expressed at higher levels in Hepa-RG cells treated with NAFLD-Exo compared to controls, after 8 and 12 h (P < 0.001) of treatment, while vimentin was expressed at significantly higher levels after 4, 8 (p < 0.005), and 12 h (p < 0.001). This evidence concerns the gene FN1 and metabolic dysfunction-associated steatotic liver disease.