Quantitation of Parp14 mRNA expression in bulk-tumour by RT-PCR analysis revealed that while expression was still significantly lower in endpoint YUMM2.1 tumours expressing shPARP14 compared to tumours expressing shNTC (Supplementary Fig. 7D), this was not the case for MC38 tumours (Supplementary Fig. 7D), suggesting a selection for elevated Parp14 mRNA expression or a loss of the shRNA in MC38 tumours treated with α-PD-1 and perhaps accounting for the less robust effect of PARP14 depletion in this model. Here, PARP14 is linked to neoplasm.