In order to exploit the 10-fold increase in yield of the more recent Sequel II instrument, we created new collections of barcoding oligos for the one-step amplification (non-nested) of polymorphic segments of msp1 and glurp,11,18 two markers used for molecular correction in malaria drug trials and recommended by the World Health Organization (WHO),8 as well as the complete open reading frame of CSP, the polymorphic liver-stage antigen in the WHO-approved vaccine RTS,S. This evidence concerns the gene ATAD1 and malaria.