We also found RNF6 amplification in dabrafenib-resistant 3D-HT-29 spheroids, which is consistent with previous studies promoting CRC progression via aberrant activation of Wnt/β-catenin signalling pathway by inhibiting GSK3β activity [53, 54]; therefore restoring the GSK3β activity might be one of the ways to overcome the resistance observed in our system. Here, RNF6 is linked to colorectal carcinoma.