HMGCS2 and acute kidney injury: In vivo, knockdown of Hmgcs2 employed the hydrodynamic gene delivery technique, and these mice were then subjected to 1-day ischemic AKI, as depicted in Figure 5, C and D. At 1 day after IRI, knockdown of Hmgcs2 aggravated Scr levels and kidney morphological changes (Figure 5, E and F, and Supplemental Figure 7J).