To decipher the molecular basis that mediates the effects of GPR65 on IEC physiology as well as colitis and chronic CAC development, we performed RNA sequencing on colonic epithelial samples from Gpr65ΔIEC and Gpr65fl/fl mice after magnetic cell sorting to exclude the contamination of CD45+ leukocytes (Supplementary Figure S7a). The gene discussed is PTPRC; the disease is colitis.