IEC-derived AMPs reinforce intestinal barrier function and are critical for the maintenance of microbiota homeostasis,33 and dysbiosis of gut microbiota contributes to the pathogenesis of colitis.34 We next performed 16S ribosomal RNA (rRNA) sequencing to characterize the fecal microbial profiles of Gpr65fl/fl and Gpr65ΔIEC mice. The gene discussed is ADSL; the disease is colitis.