CHSY1 and neoplasm: To confirm the anti-tumor effect of knockdown CHSY1 combined with anti-PD1 in vivo, MC38 cells were injected into the spleen of C57BL/6 mice in the sh-CHSY1 and sh-NC groups, respectively, followed by anti-PD1 injection to assess the liver metastatic ability of these cells (Fig. 5A).