To elucidate the role of Notum in GC pathogenesis, we carried out gene set enrichment analysis (GSEA) using public datasets (GSE65801), and the results showed that high Notum expression was positively associated with Wnt/β-catenin and PI3K/AKT signaling pathways, which was further confirmed by analysis of The Cancer Genome Atlas (TCGA) database (Additional file 1: Fig. S5A-5D). The gene discussed is AKT1; the disease is gastric cancer.