ZEB1 and colorectal carcinoma: In addition, we further experimentally validated the binding of ZEB1 to this region by electrophoretic mobility shift assays (EMSA) and observed that the rs4810856[C] allele exhibited a preferential binding to nuclear extracts compared with the rs4810856[T] allele in CRC SW480 and HCT116 cells (Figs. 4h and S10b).