TGFBR2 and colorectal carcinoma: To investigate the role of TME-derived THBS1 in aggressive CRC, we performed the orthotopic implantation of mouse tumor organoids (MTO) that had a CMS4-like mesenchymal phenotype, harboring mutations in Apc, Trp53, Kras, and Tgfbr2 in the mouse rectum17.