The brains of mice with clinical end-stage murine prion disease inoculated with CWD-exposed cervid PrP Drosophila head homogenate contained Proteinase K-resistant PrP27–30 (Figure 4b) and RT-QuIC prion seeding activity (Figure 4c), which confirmed the presence of prion disease in these animals. This evidence concerns the gene PRNP and prion disease.