The volcano plot of DEG analysis is presented in Figure 2A. The 40 most significant genes among groups are displayed in order of correlations of their Gene Counts between samples and are visualized using the heat map in Figure 2B. We found that the significant up-regulated genes in myopia samples were KIAA1211 and CALR3, and the significant down-regulated genes were DNASE2B and CCDC178. The gene discussed is CRACD; the disease is myopia.