To determine the mechanism by which downregulation of RCN1 decreases the viability of AML cells, transcriptome analysis was performed on MOLM‐13‐23, MOLM‐13‐242, RCN1‐deficient NB4, and OCI/AML3 cells, along with their respective control cells. This evidence concerns the gene RCN1 and acute myeloid leukemia.