Strikingly, moderately silencing MLCK or mDia1 considerably enhanced the colony formation in SFA and soft agar by 35 to 130% and ~65% (Fig. 1B and C), promoted the growth of tumor spheroids in soft fibrin by 80 to 140% (Fig. 1D), and elevated the fractions of EpCAM+, CD90+, and CD133+ cells by 20 to 34%, 130 to 230%, and 46 to 64%, respectively (Fig. 1E), all of which labeled liver CSCs [25,28,29]. Here, PROM1 is linked to neoplasm.