POU5F1 and neoplasm: The activity of cytoskeleton/APC/β-catenin/Oct4 signaling was examined in patient-derived tumor tissues with low, median, and high differentiation states, which were histologically graded based on hematoxylin and eosin (H&E) staining and biopsy observation according to Edmondson–Steiner grade (Fig. 6A).