Multivariate analysis indicated that diagnosis pattern (recurrent vs. primary, HR: 2.34, 95% CI: 1.43-3.85, p=0.001), histology (signet-ring cell carcinoma vs. adenocarcinoma, HR: 1.88, 95% CI: 1.07-3.3, p=0.027), VEGFA (≥179.1 vs. <179.1, HR: 2.49, 95% CI: 1.51-4.11, p<0.001) and SII (≥376.9 vs. <376.9, HR: 1.98, 95% CI: 1.10-3.56, p=0.023) were independent prognostic factors for PFS in HER2-negative AGC patients (Table S3, Figure 5A). This evidence concerns the gene ERBB2 and signet ring cell carcinoma.