For example, in RNA pull-down assays and mass spectrometry detection, circLIFR overexpression has been shown to up-regulate several regulatory genes, including VEGF. circLIFR and TANK-binding kinase (TBK1) up-regulate the expression of MMP13, MMP3, and VEGF, which promote HCC proliferation, invasion, and metastasis (81). Here, VEGFA is linked to hepatocellular carcinoma.