In addition, Roghayeh et al. reported that culturing breast cancer cells using senescent cell culture supernatant and fibroblast supernatant can downregulate the expression of stem markers such as OCT4, SOX2, NANOG, and C-MYC, result in enhanced differentiation of breast cancer cells (Pourbagher et al., 2020). This evidence concerns the gene NANOG and breast carcinoma.