In the present study, we confirmed that several VSD-related lncRNAs (GATA3-AS1, LINC01551, and LINC00598) were consistent in both maternal plasma and amniotic fluid, suggesting that the amniotic fluid cell-free lncRNAs could be released into the maternal circulation and investigated as potential markers for CHDs. Here, GATA3 is linked to ventricular septal defect.