U2AF1 and alkaline phosphatase measurement: Studies conducted using a targeted panel of 137 genes on the cohort of 259 patients with pre-PMF, overt-PMF, and PMF-AP/BP have shown that the most recurrent additional mutations were in ASXL1, TET2, SRSF2, U2AF1, and SETBP1. Mutations in ASXL1 and U2AF1 were more frequent in overt-PMF than those in pre-PMF.