ERBB2, KRAS, SMAD4, and TACC3 gene alterations were predominantly observed in EAC, whereas CCDN1, NFE2L2, FGF19, FGF3, FGF4, NOTCH1, and CDKN2B gene alterations were mostly observed in ESCC. This evidence concerns the gene NFE2L2 and esophageal squamous cell carcinoma.