ERBB2, KRAS, SMAD4, and TACC3 gene alterations were predominantly observed in EAC, whereas CCDN1, NFE2L2, FGF19, FGF3, FGF4, NOTCH1, and CDKN2B gene alterations were mostly observed in ESCC. Here, NOTCH1 is linked to esophageal squamous cell carcinoma.