BUB1 and neoplasm: Figure 5C shows the ability to predict the 1-year, 3-year and 5-year DFS probability of THCA patients. Most importantly, by combining the T stage and BUB1, the calibration curves confirmed that the 1-, 3- and 5-year survival probability predicted by the nomogram (red, yellow, grey) is close to the actual survival (Figure 5D). To validate the expression in practice, twenty paired tissue samples were collected from clinical patients at our center. As shown in Figure 5E, BUB1 was differentially expressed in tumor tissue compared to normal thyroid tissue.