GBF1 and hemolytic-uremic syndrome: Yet, it is unlikely that the mutations in DCB and HUS described above compromise GBF1/Gea1/2p function by affecting dimerization, because mutations within the DCB domain of GBF1 that prevent HUS binding and dimerization (K91A/E130A) do not affect GBF1 membrane association or GBF1 function in Golgi homeostasis and secretion (Ramaen et al., 2007; Bhatt et al., 2016).