To validate the data, an ELISA was performed on the conditioned media from CAFs activated by oral cancer cell EVs or TGFβ, displaying CAFs activated by Cal 27 or SCC-9-derived EVs to secrete upregulated levels of IL-8 and CXCL5 compared to PBS treated fibroblasts (Figures 5G, H). Here, CXCL5 is linked to lip and oral cavity carcinoma.