Compared with the MCAO group, the expression of PSD-95 in the CA1 area and that of SYN and GAP-43 positive cells in the CA3 region of rats from the TSTT and DON groups were significantly increased (p < 0.01), suggesting that ischemia leads to a decrease in the expression of PSD-95 in the hippocampal CA1 region and that of SYN and GAP-43 in the CA3 region, and the expression of synaptic-related proteins can be upregulated after TSTT treatment. This evidence concerns the gene DLG4 and ischemia.