Low-intensity pulsed ultrasound (LIPUS) of 0.5 W/cm2 ameliorates myocardial ischemia/reperfusion (MI/R) injury by alleviating cardiac hypoxic responses, oxidative stress, and inflammatory reaction with the apoptosis signal-regulating kinase 1 (ASK1)/c-Jun N-terminal kinase (JNK) pathway involved. The gene discussed is MAP3K5; the disease is myocardial ischemia.