Using bulk RNA sequencing (RNA-seq) and single-cell RNA-seq (scRNA-seq) data, as well as basic experiments, we investigated the expression of KIF18B in NPC and assessed its association with clinical outcomes, tumorigenic mechanisms, immune escape, epithelial-mesenchymal transition (EMT), N6-methyladenosine (m6A) modification, and therapeutic responses. This evidence concerns the gene KIF18B and nasopharyngeal carcinoma.