In some investigations, 6- to 8-week-old male mice (weighing approximately 25-30 g) were used to develop acute mouse models of CCL4 induction (190, 191), in which olive or corn oil served as diluents to solubilize CCL4 at ratios ranging from 10% (v/v) to 50% (v/v), and CCL4 doses of 2 mL/kg or higher can induce acute liver failure in mice (191–194). Here, CCL4 is linked to acute liver failure.