In the current study, by performing targeted bisulfite-seq analyses of 2 cisplatin-resistant RCC cell lines, including 786-O and A-498, and the normal renal tubular epithelial cell line HK-2, methylation levels of the MCL1, IGF1R, CCND1, and PTEN were validated. The gene discussed is IGF1R; the disease is renal cell carcinoma.