CHRM3 and glioblastoma: To clarify the pathological roles of CHRM3 in GBM development, we firstly studied the levels of CHRM3 in an array of cell lines, RT-PCR and WB results displayed that compared to A172 and U251-MG cells, the levels of CHRM3 were relatively higher in U87-MG and T98G (Fig. 2A).