This was motivated by the following rationale: (i) the mbf1Δ/Δ mutant shows a decrease of virulence during mouse infections and thus could help the identification of MBF1-dependent genes under these conditions; (ii) it is well-established that regulatory circuits of transcription can differ considerably between in vitro and in vivo conditions (Kumamoto, 2008; Fanning et al., 2012). Here, EDF1 is linked to infection.