ANXA5 and cancer: Analysis of cancer cell death using the detection of phosphatidylserine by Annexin V and membrane permeabilization by the accumulation of 7-aminoactinomycin D (7-AAD) dye indicated that derivative 5 decreased the number of viable cells and increased the number of apoptotic cells, particularly in the case of the Mia PaCa-2 cell line: derivative 5 at a 5 μg/mL concentration elevated the total fraction of apoptotic cells from 20% to over 70% after 48 h of treatment (Fig. 5B).