To investigate the tumor-infiltrating immune cells induced by intratumoral CV-B5/F administration, we established a humanized animal model based on β2m knockout B-NDG mice pre-injected with human peripheral blood mononuclear cells (PBMC) bearing NCI-H1299-luc xenografts and treated them with five consecutive injections of CV-B5/F.22 CV-B5/F administration suppressed tumor growth and increased the accumulation of CD3-, CD4-, CD8-, and Granzyme B positive cells (Fig. 1l, m, and Supplementary Fig. 1h- l). Here, CD4 is linked to neoplasm.