To better explore the relationship between the dramatic cytoplasm reduction of Dp427m we found in all DMD sample types, we assayed by duplex RNAscope/immunocytochemistry (ICC) control (WT1) and DMD myoblasts (patients DMD1, DMD2, DMD3, and DMD5) using the 37–42 or 63–75 probes and nucleolin antibody (Fig. 10). This evidence concerns the gene WT1 and Duchenne muscular dystrophy.