NUCLEOLIN and Duchenne muscular dystrophy: To better explore the relationship between the dramatic cytoplasm reduction of Dp427m we found in all DMD sample types, we assayed by duplex RNAscope/immunocytochemistry (ICC) control (WT1) and DMD myoblasts (patients DMD1, DMD2, DMD3, and DMD5) using the 37–42 or 63–75 probes and nucleolin antibody (Fig. 10).