Allosteric modulators, especially BAMs, not only have receptor subtype selectivity but also have the ability to control receptor signaling pathways.19,45 In our previous study, we have identified that the compound 9n performed as a Gi-biased allosteric modulator for HCAR2 and enhanced the anti-inflammatory effects in mouse model of colitis when used alongside niacin.9 However, the modulation effect of compound 9n with subtype-specific MK-6892 was still unknown. The gene discussed is GNAI1; the disease is colitis.