Moreover, histopathological examination of the tumor and vital organs (heart, liver, spleen, lungs, and kidney) with H&E staining revealed that the anti-tumor activity of C820 NPs + laser treatment was optimal and did not show any apparent pathological damage or lesion, and serum biochemical indicator (CK-MB for the heart, ALT/AST for the liver, and BUN/CRE for the kidney) assays showed normal fluctuations in these groups, suggesting that C820 NPs are biocompatible without causing any evident toxicity to the major organs (Fig. S5E-I). This evidence concerns the gene GPT and neoplasm.