Using phosphoflow cytometry to determine signaling response to IFN-γ, IFN-α, and IFN-β, phosphorylated STAT1 (p-STAT1) was reduced in lymphocytes stimulated only by IFN-α in melanoma patients compared to healthy controls, with these differences observed in CD8+ and CD4+ T cells, but not B cells. This evidence concerns the gene IFNG and melanoma.