Compared with normal tissues, the expression of ANLN, CKS2, DLGAP5, KIF14, KPNA2, MELK, NEK2, PTTG1, RACGAP1, and TOP2A was upregulated, while ERP27 expression was downregulated in pancreatic cancer tissues (P < 0.05) (Fig. S4). This evidence concerns the gene NEK2 and familial pancreatic carcinoma.