IDH1 and colon adenocarcinoma: We first cloned IDH1-R132H into the lentiviral plasmid pLV-EF1α-FLAG-IRES-Puro and produced pLV-EF1α-IDH1-R132H-FLAG-IRES-Puro lentiviruses, which were used to infect CT26 colon adenocarcinoma cells.