APP and hyperthyroidism: To best investigate the role of hyperthyroidism in affecting cognitive function and microglia polarization, as well as necroptosis, a long-term hyperthyroidism of GD mouse model were established through injecting recombinant adenovirus overexpressing thyrotropin receptor A subunit 289 (Ad-TSHR289) in AD model mice (APP/PS1) [32–34].