Subsequent analysis revealed that the top DEGs in microglial cells, such as chemokine (C-C motif) receptor 5 (Ccr5), integrin alpha-M (Itgam), colony-stimulating factor 1 receptor (CSF1R), and interleukin-6 receptor subunit alpha (Il6ra), which are implicated in AD, were involved in inflammation or microglial development (Fig. 3I and Additional file 1: Fig S3C) [45–48]. The gene discussed is IL6R; the disease is Alzheimer disease.