We used two machine learning algorithms, LASSO and SVM-RFE, to screen myopia-related biomarkers in GSE151631: the LASSO regression algorithm selected six potential biomarkers (Fig. 4A) (NR1D1, PPP1R18, RTKN, LMOD1, PGBD2, and PPP1R3D); the SVM-RFE algorithm screened also 6 potential biomarkers (Fig. 4B) were obtained (PPP1R18, NUPR1, NR1D1, PPP1R3D, PGBD2, and ZNF780A). The gene discussed is NUPR1; the disease is myopia.