HGF and steatosis: To determine if media composition of fat and/or sugar plays a role in PHH steatosis induction and reversal, MPCC plates from three donors (TWJ, LFQ, and EUJ) were treated with either 0.5 mM FFA (FFA), 10 g/L glucose + 1.0 g/L fructose (HGF), or a combination of both (FFA + HGF).