CD276 and neoplasm: To do this, due to the undetectable tumors in B12(VHH)-CAR-T treated IMR5 tumor-bearing mice, we isolated long-acting B7-H3 CAR-T cells from the spleens at week 5 after CAR-T cell infusion, followed by ex vivo tumor (IMR32 and IMR32 B7-H3 KO) incubation, and a single-cell-based 32-plex cytokines/chemokines microfluidics device was subsequently performed (Fig. 7a).