Compared to the control CD19 CAR-T cells that bind an irrelevant antigen, the B7-H3 CAR-T cells significantly lysed four NB cell lines (IMR32, IMR5, NBEB, and LAN-1), two PDAC cell lines (Panc-1 and BxPC-3), a triple-negative breast cancer (TNBC) cell line (MDA-MB-231), and a lung adenocarcinoma (LUAD) cell line (H226), but not IMR32 B7-H3 KO cells, suggesting that their cytotoxicity is antigen-dependent (Fig. 4b, c and Supplementary Fig. 4B). The gene discussed is CD276; the disease is triple-negative breast carcinoma.