CD276 and neoplasm: To validate this, we designed a pair of B7-H3 specific primers and demonstrated that both 4Ig and 2Ig transcripts exist in B7-H3 positive (B7-H3+) tumor cell lines (Panc-1, IMR5, IMR32, 293T, and HepG2) rather than B7-H3 negative (B7-H3-) lymphoma cell lines (Raji, Jurkat, and Daudi) (Fig. 1e).