Single-cell RNA sequencing (scRNA-seq) of human primary HCC tumorspheres indicated that SCARB2 positive cells accounted about 11.01% of total tumorspheres (Fig. 3e, f) and exhibited higher level of MYC target genes compared to SCARB2 negative cells (Fig. 3g–i). The gene discussed is SCARB2; the disease is hepatocellular carcinoma.