We further validated the  CUT&Tag result with ChIP-qPCR: SCARB2 knockout in HCCLM3 cells or Scarb2 knockout in mouse HCC cells in vivo reduced the binding of MYC to MYC target genes, including CAD, CDK4, LDHA, NCL, PKM2 and HES1 (Fig. 3o, p). This evidence concerns the gene CAD and hepatocellular carcinoma.