In order to determine the potential biological functions of PRMT1-mediated BRD4 methylation in vivo, we performed xenograft mouse assays with endogenous BRD4 knockdown by shBRD4 infection while expressing ectopic WT or R3K mutant BRD4 cells (A2780-shBRD4-BRD4-WT cells and A2780-shBRD4-BRD4-R3K cells). This evidence concerns the gene PRMT1 and infection.