Even though some studies have suggested that CDC27 promoted metastasis development in CRCs16 and might be a potential prognostic indicator4,17 and a therapeutic target,4,16,17 there is insufficient literature data on its association with other mutations involved in colorectal carcinogenesis.17 CDC27 mutations, which our study found significantly associated with APC, PTEN, MLH3, MSH3, and BRCA2 mutations, seem to play an important role in CRC carcinogenesis. This evidence concerns the gene MSH3 and medical procedure.