LGR5 and colitis: ,26 Retrospective analysis of RNA-seq data collected from the colonic mucosa at various time points during DSS-induced colitis validated the loss of Muc2 and Lgr5 early (d 3–6) during DSS exposure, followed by the upregulation of Ly6a (d 6) and peak expression of Tnfr2 at d 9 (Figure 1A).