Based on the above-mentioned benefits of NLC for breast cancer targeting, the present study aimed to prepare and evaluate Propolis Extract (ProE) loaded NLC for improved targeting to different inflammatory, angiogenic, proliferation, and apoptotic markers as well as, mTOR and miR-223 related pathways in Ehrlich Ascites Carcinoma (EAC) bearing mice. The gene discussed is MTOR; the disease is breast cancer.