To explore the specific mechanism of Sp1/Sp3 ablation causing endothelial dysfunction and hypertension, we used transcriptomic analysis of MLECs from CT R and dKO mice and identified 2683 genes with altered expression upon Sp1/Sp3 deletion (1591 upregulated and 1092 downregulated, p < 0.05 and |Log2FoldChange| > 1.5) (Fig. S2A). Here, SP3 is linked to endothelial dysfunction.