Besides, notch-1 and jagged1 proteins were tested, and the result revealed that RGS1 overexpression may inhibit the notch-1/jagged1 signaling pathway’s expression in ccRCC, exerting a certain effect on tumor-associated macrophage polarization (Fig. 8E, F). This evidence concerns the gene NOTCH1 and nonpapillary renal cell carcinoma.