FAT4 expression was associated with several signaling pathways in HCC, including the PI3K/AKT/mTOR pathway, ECM degradation, transforming growth factor β (TGF-β), ECM-related genes, the IL-10 anti-inflammatory signaling pathway, epithelial–mesenchymal transition (EMT) markers, collagen formation, apoptosis, p53 pathway, angiogenesis, and inflammatory response (Fig. 4a,b,c,d,e,f,g,h,i,j,k). This evidence concerns the gene TP53 and hepatocellular carcinoma.